Coding

Part:BBa_K4733007:Design

Designed by: Diego Perez Hidalgo   Group: iGEM23_UBC-Okanagan   (2023-10-05)


Photo-cleavable Linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The UBC-Okanagan 2023 team used Modular Cloning (MoClo) standardization for this part: added Type II restriction sites and standard flanks to this part.


Source

The photo-cleavage site in this part was sourced from the paper Genetically Encoded Photocleavable Linkers for Patterned Protein Release from Biomaterials by Shadish et al. (2019). The rigid linker was sourced from Chen et al. (2013).

References

Shadish, J. A., Strange, A. C., & DeForest, C. A. (2019). Genetically Encoded Photocleavable Linkers for Patterned Protein Release from Biomaterials. Journal of the American Chemical Society, 141(39), 15619–15625. https://doi.org/10.1021/jacs.9b07239

Chen, X., Zaro, J. L., & Shen, W.-C. (2013). Fusion protein linkers: Property, design, and functionality. Advanced Drug Delivery Reviews, 65(10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039